Fetal Bovine Serum
Serum is the blood component obtained after coagulation and removal of cellular components， containing growth factors, amino acids and hormones, etc. This comprehensive mix makes serum one of the most important supplements for supporting cell growth and proliferation in vitro cell culture. Of special interest is fetal bovine serum (FBS)，because it is rich in growth factors and low in antibodies, which may influence the cell culture work.
For standard cell culture applications in academic research FBS from Elite Biotech is perfectly suitable. It is manufactured by altering certain parameters, to promo-te optimal cell growth with low batch-to-batch variation, mainly from EU-approved material.
Our FBS is available from three different origins. Besides heat inactivation (HI), additional treatments such as gamma irradiation, delipidation, charcoal-stripping, dialysis, ES cell-pretesting are available (on request).
Only serum batches passing our strict quality control are released for sale. Standard parameters are determined including pH, osmolality, content of protein, albumin, IgG and hemoglobin, endotoxin level, sterility, mycoplasma detection and virus testing.
We wish to serve our customers with best of our FBS products.
Elite Biotech's Bestsellers:
Product | Origin | Volume | Cat. No. |
Fetal Bovine Serum Premium | South America | 100 ml / 500 ml | FBS-Premium-S / L |
Fetal Bovine Serum Superior | South America | 100 ml / 500 ml | FBS-Superior-S / L |
Fetal Bovine Serum AUS | Australia | 100 ml / 500 ml | FBS-AUS-S / L |
Fetal Bovine Serum AUS Superior | Australia | 100 ml / 500 ml | FBS-AUS-Superior-S / L |
Benchmark to Heat Inactivation
Heat inactivation deactivates the complement system, antibodies and other active enzymes. This must be done in a carefully controlled process in order to maintain the cell growth-promoting properties of the serum while avoiding the formation of unwanted precipitates. The process involves heating the serum in a shaking water bath at exactly +56°C with occasional shaking for 30 minutes. After that, the serum must be cooled to room temperature immediately to avoid an over-exposure to heat, which can damage growth factors and vitamins, etc.
Since heat inactivation has largely a deleterious effect, considering serum as a comprehensive mix of important supplements for supporting cell growth and proliferation. It would thus be strongly advisory to test your cell system and make sure whether a heat inactivation is truly necessary or not. Available information can be acquired from ATCC sites, publications, experiences, etc.
Note:
scientists from German Cancer Research Center (Deusches Krebs Forschungszentrum, DKFZ), please cite 35422 when place an order via internal "BANF" system for convenience.